Reconstruction of the central carbohydrate metabolism of Thermoproteus tenax by use of genomic and biochemical data.

DNA microarray analysis of central carbohydrate metabolism: glycolytic/gluconeogenic carbon switch in the hyperthermophilic crenarchaeum Thermoproteus tenax.

In order to unravel the role of regulation on transcript level in central carbohydrate metabolism (CCM) of Thermoproteus tenax, a focused DNA microarray was constructed by using 85 open reading frames involved in CCM. A transcriptional analysis comparing heterotrophic growth on glucose versus autotrophic growth on CO2-H2 was performed.

DNA Microarray Analysis of the Central Carbohydrate Metabolism : 1 Glycolytic / Gluconeogenic Carbon Switch in the Hyperthermophilic Crenarchaeum 2 Thermoproteus tenax

Embden-Meyerhof-Parnas and Entner-Doudoroff pathways in Thermoproteus tenax: metabolic parallelism or specific adaptation?

Genome data as well as biochemical studies have indicated that--as a peculiarity within hyperthermophilic Archaea--Thermoproteus tenax uses three different pathways for glucose metabolism, a variant of the reversible EMP (Embden-Meyerhof-Parnas) pathway and two different modifications of the ED (Entner-Doudoroff) pathway, a non-phosphorylative and a semi-phosphorylative version. An overview of ...

The semi-phosphorylative Entner-Doudoroff pathway in hyperthermophilic archaea: a re-evaluation.

Biochemical studies have suggested that, in hyperthermophilic archaea, the metabolic conversion of glucose via the ED (Entner-Doudoroff) pathway generally proceeds via a non-phosphorylative variant. A key enzyme of the non-phosphorylating ED pathway of Sulfolobus solfataricus, KDG (2-keto-3-deoxygluconate) aldolase, has been cloned and characterized previously. In the present study, a comparati...

Biochemical changes in carbohydrate metabolism of the fish – Cyprinus carpio during sub-lethal exposure to biopesticide – Derisom.

The effect of biopesticide - Derisom on certain metabolites and enzymes of carbohydrate metabolism were evaluated in gill, liver, kidney and muscle of Cyprinus carpio during sub-lethal toxicity exposure of 21 days. A dose of 0.28 ppm was taken as the sub-lethal dose. The organs were taken from exposed and control fish at the end of 24 hrs, 7, 14 and 21 days and used for the estimation of total ...